Genetic immunization of vaccine plasmids. (A)Western blot analysis of protein expression of vaccine DNA in transfected COS-7 cells. Lane 1 and 2, lysate of transfected COS-7 cells with pcDNA3.1/HTNV-IL-2-G2 were incubated with mouse anti-IL-2 and anti-G2 Mab, Lane 3 and 4, lysate of IL-2 and G2 protein with mouse anti-IL-2 antibody and mouse anti-G2 Mab, respectively. Lane 5 and 6, lysate of transfected COS-7 cells with pcDNA3.1(+)were incubated with mouse anti-IL-2 antibody and mouse anti-G2 Mab, respectively. M, molecular weight marker proteins: the mobility of size standards (in kDa) is shown to the left. (B) Indirect immunofluorescence of COS-7 cells transfected by recombinant vector pcDNA3.1/HTNV-IL-2-G2 (B-1) and pcDNA3.1(+) (B-2), incubated with mouse anti-G2 Mab, bridged by avidin-conjugated anti-goat IgG, and detected by rodamin conjugated biotin and FITC conjugated anti-mouse IgG, followed by laser confocal scanning.