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Figure 4 | Genetic Vaccines and Therapy

Figure 4

From: A DNA vaccine against tuberculosis based on the 65 kDa heat-shock protein differentially activates human macrophages and dendritic cells

Figure 4

Activation of adaptive immune response induced by DNA-HSP65. (A) Expression of Hsp65 mRNA by monocytes stimulated with DNA vaccine or vector was evaluated by RT-PCR. (B) Proliferation of PBMCs after stimulation with DNA-HSP65. CFSE-labeled PBMCs were cultured with DNA vaccine, vector or with recombinant Hsp65 (rHsp65). Cell proliferation was determined by flow cytometry. Results represent the mean ± SEM of nine experiments. * p < 0,05, when compared to unstimulated cells. (C) Representative histograms of PBMCs proliferation assay. Black line: stimulated cells; dotted line: unstimulated cells.

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Genetic Vaccines and Therapy

ISSN: 1479-0556