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Figure 5 | Genetic Vaccines and Therapy

Figure 5

From: Organ distribution of transgene expression following intranasal mucosal delivery of recombinant replication-defective adenovirus gene transfer vector

Figure 5

PCR amplification of an adenoviral genomic sequence after intranasal Ad inoculation. After intranasal delivery of AdLuc, mice were sacrificed at day 3 and the total DNA was isolated from organs. PCR amplification of adenoviral genomic sequences and the GAPDH control was performed using the isolated DNA as the template and primers outlined in the Methods. The PCR reactions were then electrophoresed through a 1% agarose gel, stained with ethidium bromide, and visualized under UV light. The data is representative of 4 mice. OB = olfactory bulb, LN = lymph node.

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