Figure 1

Effects of the Igκ MAR in three lentiviral vectors. Three types of cells (HEK293T, Hela and NIH-3T3) were transduced in triplicate with a series of doses of the vector (ranging from 0.1 to 15 ng of p24, measured by ELISA), and luciferase activity was measured 72 hours later. HEK293T, Hela and NIH-3T3 cells were seeded at densities of 15,000, 5,000 and 6,500 cells per well, respectively, in 96-well plates. SIN integrating (IN WT) lentiviral vectors bearing a CMV-LUC expression cassette, double-copy (DC) integrating lentiviral vectors bearing an EF1-LUC expression cassette and non-integrating (IN N) lentiviral vectors bearing a CMV-LUC expression cassette were used without (white columns) or with (black columns) the Igκ MAR. Statistical analysis was performed using a two-way ANOVA, and statistical significance of the Bonferroni post-test is represented on the relevant bars (* for p < 0.05, ** for p < 0.01 and *** for p < 0.001).