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Figure 3 | Genetic Vaccines and Therapy

Figure 3

From: Plasmid-encoded NP73-102 modulates atrial natriuretic peptide receptor signaling and plays a critical role in inducing tolerogenic dendritic cells

Figure 3

Modulation of NPRA signaling in human monocyte-derived dendritic cells (hmDCs) alters Treg generation in vitro. (A) Flow cytometry assay for Foxp3-positive T cells in naïve CD4+ CD25-T cells co-cultured with plasmid-transfected hmDCs. Results shown are from one representative experiment of three repeats. (B) RT-PCR analysis of Foxp3 expression in naïve CD4+ T cells after co-culture with plasmid-transfected hmDCs. (C) Autologous CD4+CD25-T cells were co-cultured with NP73-102-induced CD4+CD25+ T cells at different ratios and the proliferation index (PI) was calculated. (D) Phagocytosis by hmDCs transfected with the various plasmids. DCs were harvested one day after transfection, incubated with FITC-dextran for 1 h at 37°C and counted by flow cytometry. For each sample, the background (mean value of fluorescence of cells exposed to FITC-dextran at 4°C) was subtracted from the mean value of fluorescence of hmDCs incubated at 37°C. (E) Flow cytometric analysis of phenotypic markers of transfected hmDCs. Results shown are from one representative experiment of three repeats.

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Genetic Vaccines and Therapy

ISSN: 1479-0556