A comparison of multiple shRNA expression methods for combinatorial RNAi

Table 1 shRNA sequences

r ^a	Target	In. ^b	Stem (sense)	Loop	Te. ^b	Len.
T	Tat 56-29	G	AAACUGCUUGUACCAAUUGCUAUUGUAAA	ACTCGAGA	G	70
V	Vif 88-29	G	UAUAUUUCAAGGAAAGCUAAGGACUGGUU	UCTCGAGU	-	69
R	Vpr 72-29	-	GGAACUUAAGAGUGAAGCUGUUAGACAUU	ACTCGAGA	-	68
U	Vpu 158-29	-	GCAAUGAGAGUGAAGGAGAAGUAUCAGCA	ACTCGAGA	-	68
O'	Off #1-29	-	AAGACAGUCCAACACACGCCACCUGUCUC	UCTCGAGU	-	68
O"	Off #2-29	-	AACAGUCUGUCAAAGGUGACCCCUGUCUC	UCTCGAGU	-	68
T ¹⁹	Tat 59-19	-	CUGCUUGUACCAAUUGCUA	UUCUCGAGA	-	50
V ¹⁹	Vif 98-19	-	GGAAAGCUAAGGACUGGUU	UCTCGAGU	-	48

^a : shRNA abbreviation
^b : In. (Initiating)/Te. (Terminating): Extra nucleotides included 5'(the first nt. transcribed) and 3' (the last nt. transcribed prior to 1 or more U termination remnants) in only some sequences (those which were also a part of an overlapping study).