Skip to main content
Figure 2 | Genetic Vaccines and Therapy

Figure 2

From: Therapeutic dendritic cell vaccine preparation using tumor RNA transfection: A promising approach for the treatment of prostate cancer

Figure 2

PSA antigen expression of mature DCs transfected with total RNA from LNCaP cells. Mature DCs were transfected by electroporation with 4 μg (IA, B, and C) and 5 μg (IIA, B, and C) or coincubated with 5 μg (IIIA, B, and C) of RNA from LNCaP cells. The electroporation conditions were 300 (IA and IIA), 400 (IB and IIB), or 500 V (IC and IIC). In all cases, the capacitance was 25 μF. The transfection by coincubation was done without preincubation (IIIA) and with 30 minutes (IIIB) or 2 hours (IIIC) of preincubation at 22°C. Photos IVA and IVB are negative transfection controls, and IVC is the positive control represented by LNCaP cells. The panel of photographs represents PSA expression detected by immunocytochemistry using an anti-PSA antibody.

Back to article page

Genetic Vaccines and Therapy

ISSN: 1479-0556