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  1. Content type: Research

    For many promising target cells (e.g.: haematopoeitic progenitors), the susceptibility to standard adeno-associated viral (AAV) vectors is low. Advancements in vector development now allows the generation of t...

    Authors: Marius Stiefelhagen, Leopold Sellner, Jürgen A Kleinschmidt, Anna Jauch, Stephanie Laufs, Frederik Wenz, W Jens Zeller, Stefan Fruehauf and Marlon R Veldwijk

    Citation: Genetic Vaccines and Therapy 2008 6:12

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  2. Content type: Research

    Application of plasmid DNA for immunization of food-producing animals established new standards of food safety. The addition of foreign products e.g. pDNA into the food chain should be carefully examined to en...

    Authors: Petr Orság, Veronika Kvardová, Milan Raška, Andrew D Miller, Miroslav Ledvina and Jaroslav Turánek

    Citation: Genetic Vaccines and Therapy 2008 6:11

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  3. Content type: Research

    In situ production of a secreted therapeutic protein is one of the major gene therapy applications. Nevertheless, the plasmatic secretion peak of transgenic protein may be deleterious in many gene therapy applica...

    Authors: Emmanuelle E Fabre, Pascal Bigey, Yves Beuzard, Daniel Scherman and Emmanuel Payen

    Citation: Genetic Vaccines and Therapy 2008 6:10

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  4. Content type: Research

    Vectors based on adeno-associated virus-8 (AAV8) have shown efficiency and efficacy for liver-directed gene therapy protocols following intravascular injection, particularly in relation to haemophilia gene the...

    Authors: Tracey Graham, Jenny McIntosh, Lorraine M Work, Amit Nathwani and Andrew H Baker

    Citation: Genetic Vaccines and Therapy 2008 6:9

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  5. Content type: Review

    Solid tumours account for 90% of all cancers. Gene therapy represents a potential new modality for their treatment. Up to now, several approaches have been developed, but the most efficient ones are the viral ...

    Authors: Ming Q Wei, Ruimei Ren, David Good and Jozef Anné

    Citation: Genetic Vaccines and Therapy 2008 6:8

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  6. Content type: Research

    Asthma is a complex disease, characterized by reversible airway obstruction, hyperresponsiveness and chronic inflammation. Principle pharmacologic treatments for asthma include bronchodilating beta2-agonists a...

    Authors: Xiaoqin Wang, Weidong Xu, Subhra Mohapatra, Xiaoyuan Kong, Xu Li, Richard F Lockey and Shyam S Mohapatra

    Citation: Genetic Vaccines and Therapy 2008 6:7

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  7. Content type: Short paper

    Bovine herpesvirus 4 (BoHV-4) is a gamma-herpesvirus with no clear disease association. A recombinant BoHV-4 (BoHV-4EGFPΔTK) expressing Green Fluorescent Protein (EGFP), was successfully used to infect F98 rat...

    Authors: Marco Redaelli, Andrea Cavaggioni, Carla Mucignat-Caretta, Sandro Cavirani, Antonio Caretta and Gaetano Donofrio

    Citation: Genetic Vaccines and Therapy 2008 6:6

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  8. Content type: Research

    It is believed that respiratory mucosal immunization triggers more effective immune protection than parenteral immunization against respiratory infection caused by viruses and intracellular bacteria. Such unde...

    Authors: Daniela Damjanovic, Xizhong Zhang, Jingyu Mu, Maria Fe Medina and Zhou Xing

    Citation: Genetic Vaccines and Therapy 2008 6:5

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  9. Content type: Short paper

    Tattooing is one of a number of DNA delivery methods which results in an efficient expression of an introduced gene in the epidermal and dermal layers of the skin. The tattoo procedure causes many minor mechan...

    Authors: Dana Pokorna, Ivonne Rubio and Martin Müller

    Citation: Genetic Vaccines and Therapy 2008 6:4

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  10. Content type: Research

    A number of reports have demonstrated that rodents immunized with DNA vaccines can produce antibodies and cellular immune responses presenting a long-lasting protective immunity. These findings have attracted ...

    Authors: Luís H Franco, Pryscilla F Wowk, Célio L Silva, Ana PF Trombone, Arlete AM Coelho-Castelo, Constance Oliver, Maria C Jamur, Edson L Moretto and Vânia LD Bonato

    Citation: Genetic Vaccines and Therapy 2008 6:3

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  11. Content type: Research

    Early prostate adenocarcinoma can be diagnosed through seric prostate-specific antigen (PSA) screenings. However, a fraction of patients progress to an incurable metastatic disease. Therefore, novel therapies ...

    Authors: Juliana M Sousa-Canavez, Flavio C Canavez, Kátia RM Leite and Luiz H Camara-Lopes

    Citation: Genetic Vaccines and Therapy 2008 6:2

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  12. Content type: Research

    Ischemic brain injury and epilepsy are common neurodegenerative diseases caused by excitotoxicity. Their pathogenesis includes microglial production of inflammatory cytokines. Our studies were designed to exam...

    Authors: Jennifer M Laing and Laure Aurelian

    Citation: Genetic Vaccines and Therapy 2008 6:1

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  13. Content type: Short paper

    Adeno-associated virus (AAV) has shown great promise as a gene transfer vector. However, the incubation time needed to attain significant levels of gene expression is often too long for some clinical applicati...

    Authors: Lourdes M Andino, Thomas J Conlon, Stacy L Porvasnik, Sanford L Boye, William W Hauswirth and Alfred S Lewin

    Citation: Genetic Vaccines and Therapy 2007 5:13

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  14. Content type: Research

    Vaccination of neonates is generally difficult due to the immaturity of the immune system and consequent higher susceptibility to tolerance induction. Genetic immunization has been described as an alternative ...

    Authors: Ana Cláudia Pelizon, Douglas R Martins, Sofia FG Zorzella, Ana Paula F Trombone, Júlio CC Lorenzi, Robson F Carvalho, Izaíra T Brandão, Arlete AM Coelho-Castelo, Célio L Silva and Alexandrina Sartori

    Citation: Genetic Vaccines and Therapy 2007 5:12

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  15. Content type: Research

    Polyethylenimine (PEI) is one of the most efficient and versatile non-viral vectors available for gene delivery. Despite many advantages over viral vectors, PEI is still limited by lower transfection efficienc...

    Authors: Stephen R Doyle and Chee Kai Chan

    Citation: Genetic Vaccines and Therapy 2007 5:11

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  16. Content type: Research

    The presence of CAR in diverse tumor types is heterogeneous with implications in tumor transduction efficiency in the context of adenoviral mediated cancer gene therapy. Preliminary studies suggest that CAR tr...

    Authors: Blanca Segura-Pacheco, Berenice Avalos, Edgar Rangel, Dora Velazquez and Gustavo Cabrera

    Citation: Genetic Vaccines and Therapy 2007 5:10

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  17. Content type: Research

    This study aimed to demonstrate that microspheres, used as delivery vehicle of DNA-Hsp65/TDM [plasmid DNA encoding heat shock protein 65 (Hsp65) coencapsulated with trehalose dimycolate (TDM) into PLGA microsp...

    Authors: Ana Paula F Trombone, Celio L Silva, Luciana P Almeida, Rogerio S Rosada, Karla M Lima, Constance Oliver, Maria C Jamur and Arlete AM Coelho-Castelo

    Citation: Genetic Vaccines and Therapy 2007 5:9

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  18. Content type: Research

    Safe and efficient vector systems for delivering antigens or immunomodulatory molecules to dendritic cells (DCs), T lymphocytes or both are considered effective means of eliciting adaptive immune responses and...

    Authors: Mauro Pistello, Laura Vannucci, Alessia Ravani, Francesca Bonci, Flavia Chiuppesi, Barbara Del Santo, Giulia Freer and Mauro Bendinelli

    Citation: Genetic Vaccines and Therapy 2007 5:8

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  19. Content type: Research

    Vaccines are considered by many to be one of the most successful medical interventions against infectious diseases. But many significant obstacles remain, such as optimizing DNA vaccines for use in humans or l...

    Authors: Eduardo DC Gonçalves, Vânia Luiza D Bonato, Denise M da Fonseca, Edson G Soares, Izaíra T Brandão, Ana Paula M Soares and Célio L Silva

    Citation: Genetic Vaccines and Therapy 2007 5:7

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  20. Content type: Research

    Respiratory syncytial virus (RSV) causes severe bronchiolitis and is a risk factor for asthma. Since there is no commercially available vaccine against RSV, a short interfering RNA against the RSV-NS1gene (siN...

    Authors: Xiaoyuan Kong, Weidong Zhang, Richard F Lockey, Alexander Auais, Giovanni Piedimonte and Shyam S Mohapatra

    Citation: Genetic Vaccines and Therapy 2007 5:4

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  21. Content type: Research

    For reasons of efficiency Escherichia coli is used today as the microbial factory for production of plasmid DNA vaccines. To avoid hazardous antibiotic resistance genes and endotoxins from plasmid systems used no...

    Authors: Gregers J Gram, Anders Fomsgaard, Mette Thorn, Søren M Madsen and Jacob Glenting

    Citation: Genetic Vaccines and Therapy 2007 5:3

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  22. Content type: Research

    The great challenges for researchers working in the field of vaccinology are optimizing DNA vaccines for use in humans or large animals and creating effective single-dose vaccines using appropriated controlled...

    Authors: Lúcia de Paula, Célio L Silva, Daniela Carlos, Camila Matias-Peres, Carlos A Sorgi, Edson G Soares, Patrícia RM Souza, Carlos RZ Bladés, Fábio CS Galleti, Vânia LD Bonato, Eduardo DC Gonçalves, Érika VG Silva and Lúcia H Faccioli

    Citation: Genetic Vaccines and Therapy 2007 5:2

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  23. Content type: Research

    Mucopolysaccharidosis type IIIA (MPS IIIA) is the most common of the mucopolysaccharidoses. The disease is caused by a deficiency of the lysosomal enzyme sulphamidase and results in the storage of the glycosam...

    Authors: Donald S Anson, Chantelle McIntyre, Belinda Thomas, Rachel Koldej, Enzo Ranieri, Ainslie Roberts, Peter R Clements, Kylie Dunning and Sharon Byers

    Citation: Genetic Vaccines and Therapy 2007 5:1

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  24. Content type: Research

    The rapid degradation of ribonucleic acids (RNA) by ubiquitous ribonucleases limits the efficacy of new therapies based on RNA molecules. Therefore, our aim was to characterize the natural ribonuclease activit...

    Authors: Jochen Probst, Sonja Brechtel, Birgit Scheel, Ingmar Hoerr, Günther Jung, Hans-Georg Rammensee and Steve Pascolo

    Citation: Genetic Vaccines and Therapy 2006 4:4

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  25. Content type: Research

    Duchenne muscular dystrophy is a fatal genetic disorder caused by dystrophin gene mutations that result in premature termination of translation and the absence of functional protein. Despite the primary dystro...

    Authors: Abbie M Fall, Russell Johnsen, Kaite Honeyman, Pat Iversen, Susan Fletcher and Stephen D Wilton

    Citation: Genetic Vaccines and Therapy 2006 4:3

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  26. Content type: Research

    Published data indicate that formulation of pDNA with cationic lipids could greatly enhance the response to a pDNA vaccine in larger mammals. The present work tested the influence of several pDNA:cationic lipi...

    Authors: Michal Margalith and Adrián Vilalta

    Citation: Genetic Vaccines and Therapy 2006 4:2

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  27. Content type: Research

    In order to assess a new strategy of DNA vaccine for a more complete understanding of its action in immune response, it is important to determine the in vivo biodistribution fate and antigen expression. In previo...

    Authors: AAM Coelho-Castelo, AP Trombone, RS Rosada, RR Santos Jr, VLD Bonato, A Sartori and CL Silva

    Citation: Genetic Vaccines and Therapy 2006 4:1

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  28. Content type: Research

    Gene targeting in vivo provides a potentially powerful method for gene analysis and gene therapy. In order to sensitively detect and accurately measure designed sequence changes, we have used a transgenic mouse s...

    Authors: Asami Ino, Yasuhiro Naito, Hiroyuki Mizuguchi, Naofumi Handa, Takao Hayakawa and Ichizo Kobayashi

    Citation: Genetic Vaccines and Therapy 2005 3:8

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  29. Content type: Research

    Anti-tumor vaccines targeting the entire tumor antigen repertoire represent an attractive immunotherapeutic approach. In the context of a phase I/II clinical trial, we vaccinated metastatic melanoma patients w...

    Authors: Jean-Philippe Carralot, Benjamin Weide, Oliver Schoor, Jochen Probst, Birgit Scheel, Regina Teufel, Ingmar Hoerr, Claus Garbe, Hans-Georg Rammensee and Steve Pascolo

    Citation: Genetic Vaccines and Therapy 2005 3:6

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  30. Content type: Research

    Several studies have shown that cell-mediated immune responses play a crucial role in controlling viral replication. As such, a candidate SARS vaccine should elicit broad CD8+ T-cell immune responses. Several ...

    Authors: Ali Azizi, Susan Aucoin, Helina Tadesse, Rita Frost, Masoud Ghorbani, Catalina Soare, Turaya Naas and Francisco Diaz-Mitoma

    Citation: Genetic Vaccines and Therapy 2005 3:7

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  31. Content type: Research

    Lung cancer has emerged as a leading cause of cancer death in the world. Non-small cell lung cancer (NSCLC) accounts for 75–80% of all lung cancers. Current therapies are ineffective, thus new approaches are n...

    Authors: Min Zhang, Xin Zhang, Chun-Xue Bai, Xian-Rang Song, Jie Chen, Lei Gao, Jie Hu, Qun-Ying Hong, Malcolm J West and Ming Q Wei

    Citation: Genetic Vaccines and Therapy 2005 3:5

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  32. Content type: Research

    The production of cell-based cancer vaccines by gene vectors encoding proteins that stimulate the immune system has advanced rapidly in model systems. We sought to develop non-viral transfection methods that c...

    Authors: Jill A Gershan, Bryon D Johnson, James Weber, Dennis W Schauer, Natalia Natalia, Stephanie Behnke, Karen Burns, Kelly W Maloney, Anne B Warwick and Rimas J Orentas

    Citation: Genetic Vaccines and Therapy 2005 3:4

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  33. Content type: Research

    Previously, antigens expressed from DNA vaccines have been fused to the VP22 protein from Herpes Simplex Virus type I in order to improve efficacy. However, the immune enhancing mechanism of VP22 is poorly und...

    Authors: Stuart D Perkins, Helen C Flick-Smith, Helen S Garmory, Angela E Essex-Lopresti, Freda K Stevenson and Robert J Phillpotts

    Citation: Genetic Vaccines and Therapy 2005 3:3

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  34. Content type: Research

    We have constructed and evaluated the utility of a helper-dependent virus vector system that is derived from Human Cytomegalovirus (HCMV). This vector is based on the herpes simplex virus (HSV) amplicon system...

    Authors: Kutubuddin Mahmood, Mark N Prichard, Gregory M Duke, George W Kemble and Richard R Spaete

    Citation: Genetic Vaccines and Therapy 2005 3:1

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  35. Content type: Research

    Targeted gene transfection remains a crucial issue to permit the real development of genetic therapy. As such, in vivo targeted transfection of specific subsets of hematopoietic stem cells might help to sustain h...

    Authors: Alain Chapel, Olivier Deas, Morad Bensidhoum, Sabine François, Moubarak Mouiseddine, Pascal Poncet, Antoine Dürrbach, Jocelyne Aigueperse, Patrick Gourmelon, Norbert C Gorin, François Hirsch and Dominique Thierry

    Citation: Genetic Vaccines and Therapy 2004 2:16

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  36. Content type: Research

    Modulation of the immune system by genetically modified lymphoma cell vaccines is of potential therapeutic value in the treatment of B cell lymphoma. However, the anti-tumor effect of any single immunogene tra...

    Authors: Oliver Ebert, Dorothee Wilbert, Peter Buttgereit, Carsten Ziske, Dimitri Flieger and Ingo GH Schmidt-Wolf

    Citation: Genetic Vaccines and Therapy 2004 2:15

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  37. Content type: Short paper

    The establishment of efficient gene delivery to target human tissue is a major obstacle for transition of gene therapy from the pre-clinical phases to the clinic. The poor long-term patency rates for coronary ...

    Authors: Lorraine M Work, Paul N Reynolds and Andrew H Baker

    Citation: Genetic Vaccines and Therapy 2004 2:14

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  38. Content type: Short paper

    Modulation of the immune system by genetically modified immunological effector cells is of potential therapeutic value in the treatment of malignancies. Interleukin-2 (IL-2) is a crucial cytokine which induces...

    Authors: Srinivas Nagaraj, Carsten Ziske and Ingo GH Schmidt-Wolf

    Citation: Genetic Vaccines and Therapy 2004 2:12

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  39. Content type: Research

    Viral promoters are used in mammalian expression vectors because they generally have strong activity in a wide variety of cells of differing tissues and species.

    Authors: Jerome S Harms, Kurt A Eakle, Lillian S Kuo, Robert D Bremel and Gary A Splitter

    Citation: Genetic Vaccines and Therapy 2004 2:11

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  40. Content type: Research

    A vaccine that elicits durable, powerful anti-HIV immunity remains an elusive goal. In these studies we tested whether multiple treatments with viral vector-delivered HIV envelope antigen (gp120), with and wit...

    Authors: Hayley J McKee, Patricia Y T'sao, Maria Vera, Puri Fortes and David S Strayer

    Citation: Genetic Vaccines and Therapy 2004 2:10

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  41. Content type: Review

    Retroviral vector-mediated gene transfer has been central to the development of gene therapy. Retroviruses have several distinct advantages over other vectors, especially when permanent gene transfer is the pr...

    Authors: Donald S Anson

    Citation: Genetic Vaccines and Therapy 2004 2:9

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  42. Content type: Research

    The need for safe and effective treatment of dengue virus (DEN), a class A agent that causes dengue hemorrhagic fever/dengue shock syndrome, has been a critical global priority. An effective vaccine for DEN is...

    Authors: Weidong Zhang, Rajeswari Singam, Gary Hellermann, Xiaoyuan Kong, Homero San Juan, Richard F Lockey, Shuen-Ju Wu, Kevin Porter and Shyam S Mohapatra

    Citation: Genetic Vaccines and Therapy 2004 2:8

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  43. Content type: Research

    Although lentiviral vectors have been widely used for in vitro and in vivo gene therapy researches, there have been few studies systematically examining various conditions that may affect the determination of the...

    Authors: Bing Zhang, Pat Metharom, Howard Jullie, Kay AO Ellem, Geoff Cleghorn, Malcolm J West and Ming Q Wei

    Citation: Genetic Vaccines and Therapy 2004 2:6

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  44. Content type: Research

    Suicide gene therapy employing the prodrug activating system Herpes simplex virus type 1 thymidine kinase (HSV-TK)/ ganciclovir (GCV) has proven to be effective in killing experimental brain tumors. In contras...

    Authors: Ariane Söling, Christian Theiß, Stephanie Jungmichel and Nikolai G Rainov

    Citation: Genetic Vaccines and Therapy 2004 2:7

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  45. Content type: Research

    In humans, overproduction of apolipoprotein B (apoB) is positively associated with premature coronary artery diseases. To reduce the levels of apoB mRNA, we have designed an apoB mRNA-specific hammerhead riboz...

    Authors: Shumei Zhong, Shihua Sun and Ba-Bie Teng

    Citation: Genetic Vaccines and Therapy 2004 2:5

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